Spatial Proteomics

BostonGene’s versatile end-to-end multiplex immunofluorescence (MxIF) pipeline offers a comprehensive overview of tissue architecture and cell-cell interactions by detecting up to 40 protein markers from a single slide.

Our pipeline also expertly manages data from CosMx™ SMI, a high-plex in situ platform capable of visualizing up to 1,000 RNA and 64 validated protein analytes.

Cell typing and quantification

Our automated machine learning-based MxIF bioinformatics pipeline identifies and quantifies cell populations within the tumor microenvironment.

Epithelial cells
B cells
T-helper cells
T-regulatory cells
CD8+ T cells
Macrophages
Macrophages and granulocytes
Granulocytes
Fibroblasts
Endothelial cells
Unclassified
Localization of cell populations

Gain insights into the spatial distribution of immune cells, tumor, stroma, and vasculature, obtaining key findings related to tumor porosity, infiltration, and distances between cells and tissue compartments. Collectively, these insights contribute to a comprehensive understanding of the cellular landscape.

Immune cells
Tumor
Stroma
Vessels
Spatial distribution of immune cells

Understanding the spatial distribution of inflammatory cells within the tumor microenvironment provides valuable insights into tumor biology and immune response.

Epithelial cells
B cells
T-helper cells
T-regulatory cells
CD8+ T cells
Macrophages
Macrophages and granulocytes
Granulocytes
Fibroblasts
Endothelial cells
Unclassified
Cell-cell interactions and cellular communities

Take a closer look at the intricacies of cell-cell interaction within the complex tumor ecosystem. Gain a detailed understanding of how cells communicate and uncover distinct communities characterized by similarities in cell types, proportions, densities, and spatial distributions.

Epithelium-enriched
T cells contacting epithelium
T cells and macrophages contacting epithelium
Macrophages contacting epithelium
Macrophages and granulocytes contacting epithelium
Epithelium near blood vessels
B cell-enriched
T-helper cell-enriched
CD8+ T cell-enriched
T cell-enriched
Macrophages contacting T-cells
Macrophage-enriched
Macrophage- and granulocyte-enriched
Granulocyte-enriched
Blood vessel-enriched
Stroma
Unclassified
Customizable panels
Laboratory-developed test markers
AR, BCl2, BCl6, CAIX, CD3, CD4, CD8, CD10, CD11b, CD11c, CD19, CD20, CD21, CD23, CD30, CD31, CD45, CD56, CD68, CD79a, CD138, CD206, CDX2, Chromogranin A, ER, ERG, Fibronectin, FoxP3, Granzyme B, HER2, HLA-DR, ICOS, Keratin 5/6, Keratin 7, Keratin 20, Ki67, NaK ATPase, Pan-Cytokeratin, PAX8, PD1, PD-L1, PR, PSA, SMA, Synaptophysin, Vimentin, TTF-1
Research use only markers
ATM, Actin beta, Catenin beta 1, Caveolin, CD14, CD34, CD38, CD39, CD40, CD44, CD45RO, CD66, CD107a, CD141, CD163, Collagen IV, E-cadherin, EpCAM, GATA3, GP100, HIF1A, Histone H3 Phospho, HLA-A, HLA-E, IDO1, IFNG, iNOS, Keratin 5, Keratin 8/18, Keratin 14, LAG3, LIF, Mac2/Galectin-3, MPO, PCNA, Podoplanin, SOX2, T-bet/TBX21, TCF-1, TFAM, TIGIT, TOX, TP63, VISTA
Qualified Akoya
Biosciences service provider
Akoya technical details
Akoya PhenoImager® HT
  • Tyramide signal amplification (TSA)

  • Low plex (typically 6 markers)

  • High throughput staining on Bond Rx autostainer

  • Slide scanner used to acquire images

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Akoya PhenoCycler®-Fusion
  • DNA barcode-conjugated primary antibodies

  • High plex (typically 30-40 markers)

  • Low throughput manual staining

  • Automated fluid control for cyclic imaging

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Learn more about Spatial Proteomics