BostonGene Spatial Proteomics

BostonGene Spatial Proteomics

CLIA-certified CAP-accredited

Multiplex immunofluorescence (MxIF) assay provides a comprehensive overview of tissue architecture by simultaneous detection of multiple markers with single-cell resolution.

Advanced analytics of tumor cells, immune active and suppressive cell infiltration, stromal component and vascularization, and cell-to-cell interactions precisely reveal the relationships between compartments.

Holistic tissue composition

Composition of malignant and microenvironment components of the tumor broken down to the following cell types

B cells
T helpers
T-regulatory cells
CD8+ T-cells
Macrophages and granulocytes
Spatial distribution and analysis of individual cells and communities

Gain insights into the spatial distribution of any cell type along with localization within the immune cells, tumor, stroma, and blood vessels. Each individual cell and community analysis includes key findings related to tumor infiltration, community density, porosity of tissue components, and distances between cells and tissue components.

Immune cells
Proliferating malignant cell characterization

Ki67 expression is strongly associated with tumor cell proliferation and growth. Detection of this widely accepted prognostic and predictive biomarker allows us to analyze spatial topography and the density of proliferating malignant cells in tumor tissue.

left picture – original MxIF image with highlighted Ki67 intensity signal
right picture – density mask of proliferating malignant cells

Cellular communities and cell-to-cell interaction

Analysis of single cell interaction with other cells through physical contact, surface receptor-ligand interaction, and cellular junctions.

Epithelium enriched
T cells in contact with Epithelium
T cells and Macrophages in contact with Epithelium
Macrophages in contact with Epithelium
Macrophages and Granulocytes in contact with Epithelium
Epithelium near blood vessels
B-cells enriched
T-helpers enriched
CD8+ T-cells enriched
T-cells enriched
Macrophages in contact with T-cells 
Macrophages enriched
Macrophages and Granulocytes enriched 
Granulocytes enriched
Blood vessels enriched
Unclassified enriched
Advanced analytics
  • Proprietary digital pathology platform
  • Machine learning-based MxIF
  • Cellular community and interaction
  • Tissue architecture examination
  • Robust preprocessing
Customization and more verified panels available
LDT markers

AR, BCl2, BCl6, CAIX, CD3, CD4, CD8, CD10, CD11b, CD11c, CD19, CD20, CD21, CD23, CD30, CD31, CD45, CD56, CD68, CD79a, CD138, CD206, CDX2, Chromogranin A, ER, ERG, Fibronectin, FoxP3, Granzyme B, HER2, HLA-DR, ICOS, Keratin 5/6, Keratin 7, Keratin 20, Ki67, NaK ATPase, Pan-Cytokeratin, PAX8, PD1, PD-L1, PR, PSA, SMA, Synaptophysin, Vimentin, TTF-1

RUO markers

ATM, Beta-actin, beta-Catenin 1, Caveolin, CD14, CD34, CD38, CD39, CD40,  CD44, CD45RO, CD66,  CD107a, CD141, CD163, Collagen IV, E-cadherin, EpCAM, GATA3, GP100, HIF1A, Histone H3 Phospho, HLA-A, HLA-E, IDO1, IFNG, iNOS, Keratin 5, Keratin 8/18, Keratin 14, LAG3, LIF, Mac2/Galectin-3, MPO, PCNA, Podoplanin, SOX2, T-bet/TBX21, TCF-1, TFAM, TIGIT, TOX, TP63, VISTA

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